OWLS Sensors - Label free biosensors

Biomolecular interaction studies

Overview

Real-time binding data is essential to understand the dynamic interactions between proteins and other biomolecules that drive and regulate biological processes. The OWLS system provides high-quality information-rich data required for comprehensive characterization of proteins and other molecules.

Applications notes

Test measurement of label-free immunosensor using BSA-antiBSA model molecule pair (GA)

Determination of TNFa concentration in human serum

Related publications

  • Bearinger JP, Vörös J, Spencer ND, Hubbel JA, Textor M, “Nanoscale Bio-Molecular Control Using EC-OWLS”, NSTI Natonech -The Nanotechnology Conference and Trade Show, Santa Clara, May 20-24, 2007
  • Van Tassel P R, “Protein adsorption kinetics: influence of substrate electric potential”, Proteins at Solid-Liquid Interfaces (Springer-Verlag), 2006, 1. Kroslak M, Sefcik J, Morbidelli M, “Temperature and pH Effects on Deposition Kinenitcs of Beta-Lactoglobulin”, AIChE 2006 Annual Meeting San Francisco Nov.12-17
  • Grandin HM, Bonderer L, Städler B, Textor M, Vörös J, “Waveguide Excitation Fluorescence Microscopy: A New Tool for Sensing and Imaging the Biointerface”, Biosensors and Bioelectronics, 21 (8): 1476-1482, 2006
  • Halter M, Gabi M, Textor M, Vörös J, Grandin HM, “Enhanced optical Waveguide Light mode spectroscopy via detection of fluorophore absorbance” Review of Scientific Instrumens, 77, 103-105, October 2006
  • Tang C, Feller L, Rossbach P, Keller B, Vörös J, Tosatti S, Textor M, “Adsorption and electrically stimulated desorption of the triblock copolymer poly(propylene sulfide-biethylene glycol)(PPS-PEG)from indium tin oxide (ITO) surfaces” Surface Science 600, (7): 1510-1517, April 2006
  • Kroslak M, “Investigation of Deposition and Adsorption on Solid-Liquid Interfaces through Optical Waveguide Lightmode Spectroscopy”, Doctoral Thesis ETH No.16580, Zurich 2006
  • C. R. Wittmer, P. R. Van Tassel, “Probing adsorbed fibronectin layer structure by kinetic analysis of monoclonal antibody binding”, Colloids and Surfaces B:Biointerfaces 4,1103-109, 2005
  • Ngankam P, Van Tassel PR, “In-situ layer-by-layer film formation kinetics under an applied voltage measured by optical waveguide lightmode spectroscopy”, Langmuir, 21, 5865-5871, 2005
  • Zhen G L, Eggli V, Voros J, Zammaretti P, Textor M, Glockshuber R, Kuennemenn E, “Immobilization of the nzyme beta-lactamase on biotin-derivatized poly(lysine)-gpoly( ethylene glycol)-coated sensor chips: A study on oriented attachment and surface activity by enzyme kinetics and in situ optical sensing”, Langmuir, 20 (24): 10464-10473, Nov 23 2004
  • Tie Y R, Ngankam A P, Van Tassel P R, “Probing macromolecular adsorbed layer structure and history dependence via the interfacial cavity function” Langmuir 20 (24): 10599-10603, Nov 23 2004
  • Kiss E, Erdelyi K, Szendro I, Vargha-Butler EI, “Adsorption and Wetting Properties of Pluronic Block Copolymers on Hydrophobic Surfaces Studied by optical Waveguide Lightmode Spectroscopy and Dynamic Tensiometric Method”, Journal of Adhesion, 80:815-829, 2004
  • Van Tassel P R, “Protein adsorption kinetics under an applied electric field”, Encyclopedia of Nanoscience and Nanotechnology (Dekker), 14126, 2004
  • Ngankam A P, Mao G Z, Van Tassel PR, “Fibronectin Adsorption onto Polyelectrolyte Multilayer Film”, Langmuir, 20 (8), 3362-3370, Apr 2004
  • Gray J J, “The interaction of proteins with solid surfaces”, Current Opinion in Structural Biology, 14 (1): 110-115, Feb 2004
  • Tie Y R, Calonder C, Van Tassel P R, “Protein adsorption: Kinetics and history dependence”, Journal of Colloid and Interface Science, 268 (1): 1-11 Dec 2003
  • Van Tassel P R, “Statistical mechanical modeling of protein adsorption” Materialwissenschaft und werkstofftechnik, 34 (12): 1129-1132, Dec 2003
  • Brusatori M A, Tie Y R, Van Tassel P R, “Protein adsorption kinetics under an applied electric filed: An optical waveguide lightmode spectroscopy study” Langmuir, 19 (12): 5089-5097, June 2003
  • Höök F, Vörös J, Rodahl M, Kurrat R, Böni P, Ramsden J J, Textor M, Spencer N D, Tengvall P, Gold J, Kasemo B, “A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/ dissipation”, Colloids and Surfaces B: Biointerfaces, 24 (2): 155-170, March 2002
  • Vörös J, Textor M, Spencer N D, “Measurement of the water-content of proteins during adsorption”, The Seventh World Congress on Biosensors Kyoto Japan 15-17 May 2002
  • Huang N P, Michel R, Voros J, Textor M, Hofer R, Rossi A, Elbert D L, Hubbell JA, Spencer N D, “Poly(L-lysine)-g-poly(ethylene glycol) layers on metal oxide surfaces: Surface-analytical characterization and resistance to serum and fibrinogen adsorption.” Langmuir 17:489-498, 2001.
  • Calonder C, Tie Y, Van Tassel PR, “History dependence of protein adsorption kinetics”, Proceedings of the National Academy of Sciences of the United States of America 98:10664-10669, Sept 2001.
  • Calonder C, Van Tassel P R, “Kinetic regimes of protein adsorption.” Langmuir 17:4392-4395, Sept 2001
  • Brusatori M A, “Protein adsorption Kinetics under and applied electric field: An Optical Waveguide Lightmode Spectroscopy study” Wayne State University, Detroit, Michigan, 2001.
  • Kenausis G H, Voros J, Elbert DL, Huang N P, Hofer R, Ruiz-Taylor L, Textor M, Hubbell J A, Spencer N D, “Poly(L-lysine)-g-poly(ethylene glycol) layers on metal oxide surfaces: Attachment mechanism and effects of polymer architecture on resistance to protein adsorption.” Journal of Physical Chemistry B 104:3298-3309, 2000
  • Lavalle Ph, Gergely C, Lustig A, Ball V, “Critical analysis of the apoferritin adsorption at solid-liquid interfaces in the framework of a particular adsorption model” Journal of Chemical Physics, Volume 113, Number 18, 8212-8224, 2000
  • Ramsden J J , “The specificity of biomolecular particle adhesion.” Colloids and Surfaces A: Physicochemical and Engineering Aspects 173:237, Nov 2000
  • Ramsden J J , “The specificity of biomolecular particle adhesion.” Colloids and Surfaces A: Physicochemical and Engineering Aspects 165 (1-3):25-38, May 2000
  • Ball V, Ramsden J J, “Analysis of hen egg white lysozyme adsorption on Si(Ti)O-2 vertical bar aqueous solution interfaces at low ionic strength: a biphasic reaction related to solution self association”, Colloids and surfaces B-Biointerfaces, 17 (2): 8194, Feb 2000
  • Guemouri L, Ogier J, Ramsden J J , “Optical properties of protein monolayers during assembly.” Journal of Chemical Physics 109:3265-3268, 1998
  • Van Tassel P R, Guemouri L, Ramsden J J, Tarjus G, Viot P, Talbot J, “A particle- level model of irreversible protein adsorption with a post adsorption transition” Journal of Colloid and interface Science, 207 (2): 317-323, Nov 1998
  • Kurrat R, Wallvaara B, Marti A, Textor M, Tengvall P, Ramsden J J, Spencer N D, “Plasma protein adsorption on titanium: comparative in situ studies using optical waveguide lightmode spectroscopy and ellipsometry”, Colloids and Surfaces B- Biointerfaces, 11 (4): 187-201, Jul 1998
  • Ramsden J J, “Towards zero-perturbation methods for invastigating biomolecular interactions”, Colloids and Surfaces A: Physico-chemical and Engineering Aspects 141, 287-294, 1998
  • Ramsden J J, “Biospecific interaction analysis using integrated optics techniques”, in “Quantitative analysis of biospecific interactions”, edited by P. Lundahl, A. Lundqvist and E. Greijer, Harwood Academic Publishers, 1998
  • Ball V, Ramsden J J, ” Influence of D(-) and L(+) tartaric acid on lysozyme adsorption onto a Si(Ti)O2 surface” Naturwissenschaften 85, 87-89, 1998
  • Ramsden J J, Némethné-Sallay M, Vörös J, Szendri I, “Integrated optical waveguide sensor for surface adsorption study” (in Hungarian) Fizikai Szemle, 281-, 1997/9
  • Clerc D, Lukosz W, “Real-time analysis of avidin adsorption with an integrated-optical output grating coupler: adsorption kinetics and optical anisotropy of adsorbed monomolecular layers”, Biosensors & Bioelectronics 12 :185-194, 1997
  • Kurrat R, Prenosil J E, Ramsden JJ, “Kinetics of human and bovine serum albumin adsorption at silica-titania surfaces”, J. Col. Int. Sci. 185, 1-8, 1997
  • Luthi P O, Ramsden J J, Chopard B, “Role of diffusion in irreversible deposition”, Physical Review E 55, 3111-3115, 1997
  • Lukosz W, “Integrated-optical and surface-plasmon sensors for directaffinity sensing. Part II: Anisotropy of adsorbed or bound protein adlayers”, Biosensors & Bioelectronics 12, 175-184, 1997
  • Clerc D, Lukosz W, “Real-time analysis of avidin adsorption with an integrated-optical output grating coupler: adsorption kenetics and optical anisotropy of adsorbed monomolecular layers”, Biosensors & Bioelectronics 12, 185-194, 1997
  • Ball V, Ramsden J J, “Absence of surface exclusion in the first stage of lysozyme adsorption is driven through electro static self-assembly”, J. Phys. Chem B, 54655469, 1997
  • Ramsden J J, Karrasch S, “Activated Langmuir-Blodgett films for immobilizing proteins on planar surfaces”, Sensors and Materials 8, 469 – 476, 1996
  • Ramsden J J, Dreier J, “Kinetics of the interaction between DNA and the type IC restriction enzyme EcoR124II”, Biochemistry 35, 3746-3753, 1996
  • Polzius R, Schneider Th, Bier F F, Bilitewski U, Koschinski W, “Optimization of biosensing using grating couplers: immobilization on tantalum oxide waveguides”, Biosensors & Bioelectronics 11, 503 – 514, 1996
  • Ramsden J J, Bachmanova G I, Archakov A I, “Immobilizationof proteins to lipid bilayers”, Biosensors & Bioelectronics 11, 523 – 528, 1996
  • Gao H, Sänger M, Luginbühl R, Sigrist H, “Immunosensing with photoimmobilized immunoreagents on planar optical wave-guides”, Biosensors & Bioelectronics 10, 317 – 328, 1995
  • Ramsden J J, Schubert Wright C, “The interaction between wheat germ agglutinin and membrane incorporated glycophorin A. An optical binding study”, Glycoconjugate Journal 12, 113-121, 1995
  • Ramsden J J, Roush DJ, Gill D S, Kurrat R, Willson R C, “Protein adsorption kinetics drastically altered by repositioning a singel charge”, J. Am. Chem. Soc. 117, 85118516, 1995
  • Ramsden J J, Prenosil J E, “The effect of ionic strength on protein adsorption kinetics”, J. Phys. Chem. 98, 5376 – 5381, 1994
  • Ramsden J J, “Experimental methods for investigating protein adsorption kinetics at surfaces”, Quarterly Reviews of Bio-physics 27, 1, 41 – 105, 1994
  • Kurrat R., Ramsden J J, Prenosil J E, “Kinetic model for serum albumin adsorption: experimental verification” J. Chem. Soc. Faraday Trans. 90, 587-590, 1994
  • Ramsden J J, Bachmanova G I, Archakov A I, “Kinetic evidence for protein clustering at a surface”, Phys. Rev. E 50, 5072-5076, 1994
  • Ramsden J J, “Concentration Scaling of Protein Deposition Kinetics”, Physical Review Letters 71, 295 – 298, 1993
  • Ramsden J J, “Calcium-dependence of laminin binding to phospholipid membranes”, Biopolymers 33, 475-477, 1993
  • Ramsden J J, “Observation of anomalous diffusion of proteins near surfaces”, J. Phys. Chem. 96, 3388, 1992
  • Ramsden J J, Roush D J, Gill D S, Kurrat R, Willson R C, “Protein adsorption kinetics drastically altered by repositioning a single charge”, J. Am. Chem. Soc 117, 8511 – 8516, 1995
  • Kurrat R, Ramsden J J, Prenosil J E, “Kinetic model for serum albumin adsorption: experimental verification”, Journal of the Chemical Society Faraday Transactions 90, 587 – 590, 1994
  • Ramsden J J, “Review of New Experimental Techniques for Investigating Random Sequential Adsorption”, Journal of Statistical Physics 73, 853 – 877, 1993
  • Ramsden J J, “Observation of anomalous diffusion of proteins near surfaces”, Journal of Physical Chemistry 96, 3388, 1992

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