OWLS Sensors - Label free biosensors

Living cells kinetics and growth spreading


The OWLS grating coupler sensors can yield the number, size and shape of living cells growing on its surface. The OWLS system allows to quantify the surface area of focal adhesion of living cells, at the surface. The measurement takes place in real time and is noninvasive. Applications include toxicology, stem cell and cancer research. With the addition of electrical field stimulation in EC-OWLS systems, living cell behaviour under electrical field can be studied.

Applications notes

PDF iconQuantification of cell adhesivity

PDF iconLabel-free immunosensor for Escherichia Coli detection

PDF iconEC-OWLS for Escherichia-Coli detection

Related publications

  • Wittmer C R, Phelps J A, Saltzman W M, Van Tassel P R, “Fibronectin terminated multilayer films: protein adsorption and cell attachment studies”, Biomaterials, 28, 851-860, 2007
  • Adányi N, Németh E, Halász A, Szendro I, Váradi M, “Application of electrochemical optical waveguide lightmode spectroscopy (EC-OWLS) for studying the effect of different stress factors on lactic acid bacteria.” ANALYTICA CHIMICA ACTA, 573: 4147 Sp. Iss. SI Jul 28 2006
  • Madarasz E, Lefkovets I, Erdelyi K, Szendro I, “Label-free OWLS assay on the kinetics of cell attachment: Quantification of cell adhesivity” Biosensors 2006, Seattle
  • Calonder C, Matthew HWT, Van Tassel PR, “Adsorbed layers of oriented fibronectin: a strategy to control surface-cell interactions”, , Journal of Biomedical Materials Research A, 75, 316, 2005, Published online 29 July 2005 in Wiley InterScience (www.interscience.wiley.com)
  • Vodouhê C, Schmittbuhl M, Boulmedais F, Bagnard D, Vautier D, Schaaf P, Egles C, Voegel J, Ogier J, “Effect of functionalization of multilayered polyelectrolyte films on motoneuron growth”, Biomaterials, 26 (5): 545-554, Feb 2005
  • Richert L, Arntz Y, Schaaf P, Voegel J C, Picart C, “pH dependent growth of poly(Llysine)/ poly(L-glutamic) acid multilayer films and their cell adhesion properties”, Surface Science, 570 (1-2): 13-19, Oct 10 2004
  • Horváth R, Pedersen HC, Skivensen N, Selmeczi D, Larsen N B, “Optical Waveguide sensor for on-line monitoring of bacteria” Optics Letters, 28 (14):1233-, 2003
  • Richert L, Lavalle P, Vautier D, Senger B, Stoltz J F, Scheaaf P, Voegel J C, Picart C “Cell interactions with polyelectrolyte multilayer films” Biomacromolecules, 3 (6): 11701178, Nov-Dec 2002
  • Hug T S, Prenosil J E, Maier P, Morbidelli M “On-line monitoring of adhesion and proliferation of cultures hepatoma cells using optical waveguide lightmode spectroscopy (OWLS), Biotechnology Progress, 18 (6): 1408-1413, Nov-Dec 2002
  • Hug T S, Prenosil J E, Maier P, Morbidelli M “Optical waveguide lightmode spectroscopy (OWLS) to monitor cell proliferation quantitatively” Biotechnology and Bioengineering, 80 (2): 213-221 Oct 2002
  • Hug T S, Prenosil J E, Morbidelli M, “Optical waveguide lightmode spectroscopy as a new method to study adhesion of anchorage-dependent cells as an indicator of metabolic state”, Biosensors and Bioelectronics 16 (9-12):865-874, Dec 2001
  • Voros J, Graf R, Kenausis GL, Bruinink A, Mayer J, Textor M, Wintermantel E, Spencer ND, “Feasibility study of an online toxicological sensor based on the optical waveguide technique. ” Biosensors and Bioelectronics 15:423. 2000.
  • Ruiz L, Fine E, Vörös J, Makohliso S A, Léonard D, Johnston D S, Textor M, Mathieu H J, “Phosorylcholine-containing polyurethanes for the control of protein adsorption and cell attachment via photoimmobilized laminin oligopeptides”,J. Biomater. Sci. Polymer Edn. 10 , 931-955, 1999
  • Ramsden J J, Li S Y, Heinzle E, Prenosil J E, “Optical method for measurement of number and shape of attached cells in real time”, Cytometry 19, 97 – 102, 1995Ramsden JJ, Li SY, Prenosil J E, Heinzle E, “Kinetics of adhesion and spreading of animal cells”, Biotechnology and Bioengineering 43, 939 – 945, 1994
  • Li S Y, Ramsden J J, Prenosil J E, Heinzle E, “Measurement of adhesion and spreading kinetics of baby hamster kidney and hybridoma cells using an integrated optical method”, Bio-technol. Prog. 10, 520 – 524, 1994
  • Gebbert A, Bier F F, Bilitewski U, Schmid R D, “Microbial Principles in Bioprocesses Cell Culture Technology-Downstream Processing and Recovery”, Dechema Biotechnology Conferences, Vol. 5, Part A edited by G. Kreysa and A.J. Driesel, 489 – 492, 1992

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