OWLS Sensors - Label free biosensors

Membrane protein–lipide bilayer interactions

Overview

In biology, the bilayer lipid membrane (BLM) is probably the most important surface with which proteins interact. The ease with which a BLM can be deposited onto a grating coupler sensor (GCS) is a major advantage of using the OWLS technology.

Related publications

  • Lukacs A, Garab G, Papp E, “Measurement of the optical parameters of purple membrane and plant light-harvesting complex films with optical waveguide lightmode spectroscopy.” Biosensors & Bioengineering, 21 (8): 1606-1612, Feb 15 2006
  • Shanshiashvili LV, Suknidze NC, Machaidze GG, Mikeladze DG, Ramsden JJ, “Adhesion and clustering of charge isomers of myelin basic protein at model myelin membranes”, Archives of Biochemistry and Biophysics, 419 (2): 170-177, November 2003
  • Horváth R, Fricsovszky G, Papp E, “Application of the optical waveguide lightmode spectroscopy to monitor lipid bilayer phase transition”, Biosensors and Bioelectronics, 18 (4): 415-428, April 2003
  • Michielin O, Vergcres G, Ramsden J J, “Myristoylation-induced compaction of a membrane-binding protein.”, Journal of the American Chemical Society. 121:65236526. 1999
  • Michielin O, Ramsden J J, Vergcres G, “Unmyristoylated MARCKS-related protein (MRP) binds to supported planar phosphatidyl-choline membranes”, Biochemica et Biophysica Acta 1375, 110-116, 1998
  • Ramsden J J, Bachmanova G I, Archakov A I, “Immobilization of proteins to lipid bilayers”, Biosensors and Bioelectronics 11, 523 – 528, 1996
  • Vergcres G, Ramsden J J, Waskell L, “The carboxyl terminus of the membrane- binding domain of cytochrome b5 spans the bilayer of the endoplasmic reticulum”, J. Biol. Chem. 270, 3414 – 3422, 1995
  • Heyse S, Vogel H, Sänger M, Sigrist H, “Covalent attachment of functionalized lipid bilayers to planar wave guides for measuring protein binding to biomimetic membranes”, Protein Science 4, 2532 – 2544, 1995
  • Ramsden J J, Bachmanova G I, Archakov A I, “Kinetic evidence for protein clustering at a surface”, Physical Review E 50, 5072 – 5076, 1994
  • Ramsden J J, Schneider P, “Membrane insertion and antibody recognition of a Glycosylphosphatidylinositol-Anchored protein: An optical study”, Biochemistry 32, 0523 -529, 1993
  • Ramsden J J, “Calcium-dependence of Laminin binding to Phospholipid membranes”, Biopolymers 33, 475 – 477, 1993
  • Ramsden J J, “Sensitivity enhancement of integrated optic sensor using Langmuir- Blodgett lipid films”, Sensors and Actuators B, 15 -16, 439 – 442, 1993

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