OWLS Sensors - Label free biosensors

Biosensors and immunosensors


Wide variety of different sensing applications from small drug molecules to large proteins. Ligand/receptor binding study is fundamental for understanding molecular recognition in biology. The whole immune system is based upon it, as well as the blood coagulation cascade, signalling transduction between and within cells.

Applications notes

PDF iconLabel-free immunosensor for HSP70 detection

PDF iconLabel-free immunosensor for herbicide trifluralin detection

PDF iconDetermination of TNFa concentration in human serum

PDF iconLabel-free immunosensor for Aflatoxin B1

Related publications

  • Grieshaber D, Reimhult E, Voros J, “Enzymatic Biosensors towards a Multiplexed Electronic Detection System for Early Cancer Diagnostics” Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 1: 402 – 405, Jan. 2007
  • Adanyi N, Varadi M, Kim N, Szendro I, “Development of immunosensors for determination of contaminants in food” available online 15 August 2005 at www.sciencedirect.com, Current Applied Physics 6 279-286, 2006
  • Adányi N, Levkovets I A, Rodriguez-Gil S, Szendrı I, Ronald A, Váradi M, “Development of immunosensor based on OWLS technique for determining aflatoxin B1 and ochratoxin A”, Biosensors 2004, 24-26 May, 2004, Granada, Spain
  • Váradi M, Adányi N, Kun Sz, Rezessy-Szabó J, Levkovets I A, Szendrı I, “Development of amperometric biosensor and of OWLS based immunosensor for determining biogenic amines in fermented juices”, Biosensors 2004, 24-26 May, 2004, Granada, Spain
  • Kim N, Park I-S, “Development of optical grating coupler biosensor immobilized with Salmonella antibody”, Biosensors 2004, 24-26 May, 2004, Granada, Spain Nagel T, Schmidt PM, Heise C, Ehrentreich-Förster E, Singh M, Bier F F, “Label free detection of tuberculosis infection in blood serum” Biosensors 2004, 24-26 May, 2004, Granada, Spain
  • Levkovets I, Adányi N, Trummer N, Váradi M, Szendro I, Starodub N F, Székács A, “Development of optical (OWLS) immunosensors for macromolecules and small analytes”, BIOKÉMIA XXVIII/1, 2004
  • Székács A, Trummer N, Adányi N, Váradi M, Szendro I, “Development of a non-labeled immunosensor for the herbicide trifluralin via optical waveguide lightmode spectroscopic detection” Analytica Chimica Acta 487 (1): 31-42, July 2003
  • Brusatori M A, Van Tassel P R, “Biosensing under an applied voltage using optical waveguide lightmode spectroscopy”, Biosensors and Bioelectronics, 18 (10): 1269- 1277, September 2003
  • Vörös J, Ramsden JJ, Csucs G, Szendrõ I, De Paul S M, Textor M, Spencer N D “Optical Grating Coupler Biosensors” Biomaterials 23 3699-3710, 2002 Trummer N, Adányi N, Váradi M, Szendro I, “Modification of the surface of integrated optical waveguide sensors for immunosensor applications” Fresenius J. Anal. Chem. 371, 2001,
  • Horvath R, Kerekgyarto T, Csucs G, Gaspar S, Illyes P, Ronto G, Papp E, “The effect of UV irrradiation on uracil thin layer measured by optical waveguide lightmode spectroscopy.” Biosensors & Bioelectronics 2000.
  • Ramsden J J, “OWLS: A versatile technique for sensing with bioarrays”, Chimia 53, 67- 71, 1999
  • Ramsden J J, “A sum parameter sensor for water quality”, Wat. Res. 33, 1147-1150, 1999
  • Szendro I, “Integrated Optical Chemical and Biochemical sensors”, Advanced Study Course on Optical Chemical Sensors, Neusiedl am See, Austria, 27-29 september 1999
  • Bilitewski U, Bier F F, Brandenburg A, “Immunobiosensors based on grating couplers”, in K. R. Rogers and A. Mulchandani, “Affinity Biosensors-Techniques and Protocols”, Human Press, Totowa, 121-134, 1998
  • Piehler J, Brandenburg A, Brecht A, Wagner E, Gauglitz G, “Characterisation of grating couplers for affinity based pesticide sensing”, Applied Optics (1997)
  • Lukosz W, “Integrated-optical and surface-plasmon sensors for direct affinity sensing. Part II: Anisotropy of adsorbend or bound protein adlayers”, Bosensors & Bioelectronics 12 , 175-184, 1997
  • Balázs J, M. Rácz, I Szendro, J. Vörös, A. Hámori and Zs. Szabó, “Development of integrated optical sensors-technology and measurement”, Proceedings of the ninth
  • Hungarian-Korean seminar: Integrated circuits and devices, edited by E. Czoboly, Budapest, 301-310, 1997
  • Piehler J, Brandenburg A, Brecht A, Wagner E, Gauglitz G, “Reflection grating coupler for affinity based pesticide detection”, Proccedings of the 11th European Conference on Solide State Transducers, Eurosensors XI, Warsaw, Poland, 1437-1440, 1997
  • Clerc D, Lukosz W, “Direct immunosensing with an integrated-optical output grating coupler”, Sensors and Actuators B40, 53-58, 1997
  • Ramsden J J, “Optical biosensors”, J. Mol. Rec., 10 109-120, 1997
  • Polzius R, Diessel E, Bier F F, Bilitewski U, ” Real-time observation of affinity reactions using grating couplers: determination of the detection limit and calculation of kinetic rate constants”, Analytical Biocemistry 248, 269-276, 1997
  • Makower A, Barmin A, Morzunova T, Eremenko A, Kurochkin I, Bier F F, Scheller F W, “Affinity enzymometric assay for detection of phosphororganic compounds”, Analytica Chemica Acta 357, 13-20, 1997
  • Brandenburg A, Polzius R, Bier F, Bilitewski U, Wagner E, “Direct observation of affinity reactions by reflected mode operation of integrated optical grating coupler”, Sensors and Actuators B 30, 55 – 59, 1996
  • Ramsden J J, “A dosimeter for oligopeptide hormones”, Sensors and Actuators B 30, 107 – 110, 1996
  • Bier F F, Stöcklin W F M, E. Ehrentreich-Förster and F.W. Scheller, “Immunosensing of 2,4-dichlorophenoxy acetic acid using biochemical amplification. Influence of organic solvent on binding observed by evanescent field technology”, Proc. SPIE 2504, 153 – 158, 1995
  • Ramsden J J, Schubert Wright C, “The interaction between wheat germ agglutinin and membrane incorporated glycophorin A. An optical binding study”, Glycoconjugate Journal 12, 113 – 121, 1995
  • Bernard A, Bosshard H R, “Real-time monitoring of anti-gen-antibody recognition on a metal oxide surface by an optical grating coupler sensor”, Eur. J. Biochem. 230, 416 – 423, 1995
  • Bier F F, Schmid R D, “Real time analysis of competitive binding using grating coupler immunosensors for pesticide detection”, Biosensors and Bioelectronics 9, 125 – 130, 1994
  • Clerc D, Lukosz W, “Integrated optical output grating coupler as bio chemical sensor”, Sensors and Actuators B, 18 – 19, 581 – 586, 1994
  • Bier F F, Jockers R, Schmid R D, “Integrated optical immunosensors for striazine determination: regeneration, calibration and limitations”, Analyst 119, 437 – 441, 1994
  • Hodgson J, “Light, Angles, Action. Instruments for label-free, real-time monitoring of intermolecular interactions”, Bio/Technology 12, January, 31 – 35 1994
  • Ramsden J J, Schneider P, “Membrane insertion and antibody recognition of a Glycosylphosphatidylinositol-Anchored protein: An optical study”, Biochemistry 32, 523 – 529, 1993
  • Jockers R, Bier FF, Schmid R D, “Specific binding of photosynthetic reaction centres to herbicide-modified grating couplers”, Analytica Chimica Acta 280, 53 – 59, 1993
  • Polzius R, Bier F F, Bilitewski U, Jäger V, Schmid R D, “On-line monitoring of monoclonal antibodies in animal cell culture using a grating coupler”, Biotechnology and Bioengineering 42, 1287 – 1292, 1993
  • Panayotou G, Waterfield M D, End P, “Riding the evanescent wave”, Current Biology 3, 913 – 915, 1993
  • Bier F F, Stöcklein W, Schmid RD, “Direct observation of antiatrazine antibody binding using grating couplers”, GBF-Monographs Vol. 17; Eds. R.D. Schmid and F. Scheller, VCH Weinheim, 205, 1992
  • Nellen Ph M, Lukosz W, “Model experiments with integrated optical input grating couplers as direct immunosensors”, Biosensors & Bioelectronics 6, 517 – 525, 1991
  • Lukosz W, Clerc D, Ph M Nellen, Stamm Ch, Weiss P, “Output grating couplers on planar optical waveguides as direct immunosensors”, Biosensors & Bioelectronics 6, 227 – 232, 1991
  • Spohn P K, Prenosil J E., Tiefenthaler K, “A novel instrumental set-up for in-situ detection of protein ad-sorption with Grating Coupler Sensors (GCS)”, Analytical Letters 23, 411 – 424, 1990
  • Nellen P M, Tiefenthaler K, Lukosz W, “Integrated Optical Input Grating Couplers as Biochemical Sensors”, Sensors and Actuators 15, 285, 1988
  • Spohn P K, Seifert M, “Interaction of aqueous solutions with Grating Couplers used as Integrated Optical Sensors and their pH behaviour”, Sensors and Actuators 15, 309, 1988
  • Seifert M, Tiefenthaler K, Heuberger K, Lukosz W, Mosbach K , “An Integrated Optical Biosensor (IOBS)”, Analytical Letters 19 (1+2), 205 – 216, 1986

Learn more about the OWLS applications